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The purpose of this lab is to extract DNA from a variety of cells and see DNA molecules (this procedure actually took scientists many years to discover). This will show that, contrary to popular opinion, DNA is not just found in blood cells, but in a variety of tissues (try at least one plant tissue and one animal tissue). Prior knowledge should include the fact that cell membranes are layers of lipids, or fat molecules, that DNA is found in the nucleus of a cell, and that enzymes speed up chemical reactions. The plant and animal cells will be chemically treated to break open the cell and nuclear membranes. The part of the cell mixture containing DNA will be separated from the cell membranes and associated proteins (gloppy portion), and the solution containing the dissolved DNA will be altered so that the DNA can no longer remain dissolved. It will be “precipitated,” and observable with the naked eye.
DNA Extraction